CJC-1295 + GHRP-2 (10mg Blend) Dosage Protocol
GHRH analog (CJC-1295) + ghrelin GH secretagogue (GHRP-2) blend — research-only; not FDA-approved, combination not clinically validated.
Mix & measure CJC-1295 + GHRP-2 · 10 mg
Pre-filled with this protocol’s recommended BAC water and documented starting dose — edit any field to run your own numbers.
Reconstitution math only — not dosing advice. U-100 syringe: 100 units = 1 mL. Full reconstitution guide → · Advanced calculator →
Quickstart Highlights
This page covers a research combined blend of CJC-1295 (a long-acting GHRH analog) and GHRP-2 (a ghrelin/GHS-R agonist) supplied together in one 10 mg blend vial. The pairing is intended to stimulate the GH axis from two directions at once — a theoretical synergy that has not been clinically validated for this co-formulation. CJC-1295 is not FDA-approved, and GHRP-2 is not FDA-approved in the US (it is approved in Japan only as a single-dose GH-deficiency diagnostic agent, not as chronic therapy). Doses below are total-blend amounts; the per-component split is not disclosed and is not invented here. Presented for research and educational use only.
Add 3.0 mL bacteriostatic water to the 10 mg blend vial → ~3.33 mg/mL (3,333 mcg/mL); 1 unit ≈ 33.3 mcg on a U-100 syringe.
150–300 mcg total blend once daily (evening), titrated gradually across a 12-week course.
At ~3.33 mg/mL, 150 mcg ≈ 4.5 units (0.045 mL) and 300 mcg ≈ 9 units (0.09 mL).
Lyophilized: refrigerate at 2–8 °C or freeze at −20 °C (−4 °F) long-term; once reconstituted, refrigerate and use within 2–4 weeks.
Important: Start with the Prep & Injection Guide — it covers the preparation and safety basics every protocol on this site assumes.
Dosing & Reconstitution Guide
One practical dilution of the combined blend, with total-blend dosing step by step
Note on doses: all amounts below are for the total combined blend and assume a 10 mg total blend reconstituted in 3.0 mL (~3.33 mg/mL). The individual CJC-1295 / GHRP-2 split is not disclosed, so per-component doses are not stated.
Standard / Gradual Approach (3 mL = ~3.33 mg/mL)
Reconstitute: Add 3.0 mL bacteriostatic water to the 10 mg blend vial → ~3.33 mg/mL (3,333 mcg/mL).
Typical daily range: 150–300 mcg total blend once daily, raised gradually over a 12-week course.
Easy measuring: At ~3.33 mg/mL, 1 unit ≈ 33.3 mcg on a U-100 syringe (units = mcg ÷ 33.3).
Storage: Lyophilized: refrigerate at 2–8 °C or freeze at −20 °C (−4 °F); after reconstitution, refrigerate and use within 2–4 weeks.
Frequency: one subcutaneous injection each evening (about 30–60 minutes before bed), to align with the body’s nocturnal GH peak[6]. These figures come from reference protocols, not from approved human dosing.
Advanced / Twice-Daily Approach (3 mL = ~3.33 mg/mL)
Some reference protocols split the daily total into two administrations (morning fasted and evening before bed) to reinforce GH pulsatility[1]. Keep doses at least 3–8 hours apart.
Reconstitution Steps
Draw 3.0 mL of bacteriostatic water into a sterile syringe.
Release it slowly down the vial’s inner wall to limit foaming.
Swirl or roll gently until fully dissolved — don’t shake.
Label with the date and concentration, then refrigerate at 2–8 °C (35.6–46.4 °F), shielded from light.
Important: This guide is for educational purposes only and is not medical advice. For research use only. Not for human consumption.
Supplies Needed
Quantities below assume an 8–16 week course of once-daily injections with gradual titration of the combined blend.
At ~300 mcg/day, a 10 mg blend vial covers roughly 4–5 weeks of dosing.
- 8 weeks: ~2 vials
- 12 weeks: ~3 vials
- 16 weeks: ~4 vials
- Per injection: 1 syringe
- 8 weeks (once daily): ~56 syringes
- 16 weeks (once daily): ~112 syringes
Use ~3.0 mL per 10 mg blend vial for reconstitution.
- 8 weeks (2 vials): ~6 mL → 1 bottle
- 16 weeks (4 vials): ~12 mL → 2 bottles
One for the vial stopper + one for the injection site each day.
- Per injection: 2 swabs
- 8 weeks (once daily): ~112 swabs → 1–2 boxes
Protocol Overview
A concise summary of the once-daily regimen for the combined blend, drawn from commonly cited reference protocols.
- ▪Goal: Dual GH-axis stimulation — CJC-1295 sustains GH/IGF-1 while GHRP-2 drives an acute GH pulse; the combined effect is theoretical and not validated for this co-formulation[1].
- ▪Schedule: Daily subcutaneous injections (evening) for 8–12 weeks, optionally extended to ~16 weeks.
- ▪Dose Range: 150–300 mcg per day (total blend) with gradual titration.
- ▪Reconstitution: 3.0 mL bacteriostatic water per 10 mg blend vial gives ~3.33 mg/mL for accurate unit measurements.
- ▪Storage: Lyophilized refrigerated or frozen; once mixed, refrigerate at 2–8 °C and avoid repeated freeze–thaw.
Dosing Protocol
A suggested daily titration approach based on common reference doses (total blend).
- ▪Start: 150 mcg once daily for Weeks 1–2 to allow acclimatization.
- ▪Target: 300 mcg once daily from Weeks 3–12.
- ▪Frequency: Once daily subcutaneously, preferably in the evening / before bed.
- ▪Cycle Length: Typically 8–12 weeks; optional extension to ~16 weeks.
- ▪Timing: Inject on an empty stomach; GHRP-2’s GH response can be blunted by elevated blood glucose[4]. Rotate injection sites.
Storage Instructions
Correct storage preserves the blend’s stability and activity[9].
- ▪Lyophilized: Refrigerate at 2–8 °C (35.6–46.4 °F); freeze at −20 °C (−4 °F) for long-term storage.
- ▪Reconstituted: Refrigerate at 2–8 °C and use within 2–4 weeks for optimal potency[10].
- ▪Handling: Let vials reach room temperature before opening to reduce condensation, and keep solution clear of heat and direct light.
- ▪Freeze–thaw: If long-term storage of the reconstituted solution is needed, aliquot and freeze once; avoid repeated freeze–thaw.
Important Notes
Practical points that keep daily administration safe and consistent.
- ▪Sterile technique: Use a fresh sterile U-100 insulin syringe each time and drop it straight into a puncture-proof sharps container afterward[7].
- ▪Site rotation: Move between abdomen, thighs and upper arms to reduce local irritation.
- ▪Empty stomach: Inject fasted; GHRP-2’s GH response may be blunted by elevated blood glucose[4].
- ▪Recordkeeping: Log the daily dose and injection site to keep the protocol consistent.
- ▪Regulatory note: Both peptides are research-only here — CJC-1295 is not FDA-approved, and GHRP-2 is not FDA-approved in the US (approved in Japan only as a single-dose GH-deficiency diagnostic). Both are WADA-prohibited (S2) at all times[6].
How This Works
CJC-1295 is a long-acting GHRH analog — a modified fragment of growth-hormone-releasing hormone engineered for an extended half-life. By providing sustained GHRH signalling, it can keep GH and IGF-1 elevated for days after a single dose[1].
GHRP-2 (pralmorelin) is a synthetic ghrelin / GH-secretagogue-receptor (GHS-R) agonist. It triggers an acute, pulsatile GH release that peaks within roughly 25 minutes of administration[3].
Delivered together as a blend, the two are intended to stimulate the GH axis through two complementary pathways at once — CJC-1295’s sustained GHRH tone plus GHRP-2’s rapid secretagogue pulse. This dual-stimulation rationale is a theoretical synergy and has not been clinically validated for this specific co-formulation.
Approval status: CJC-1295 is not FDA-approved (its development was discontinued after Phase 2). GHRP-2 is not FDA-approved in the US, but is approved in Japan — only as a single-dose diagnostic agent for assessing growth-hormone deficiency, not as a chronic therapy.
Both peptides are presented here as unapproved research chemicals for research and educational purposes only; the combination is not a medicine and is not clinically validated.
Lifestyle Factors
Habits that may complement GH-axis activity alongside the protocol.
- ▪Nutrition: Keep a balanced, protein-forward diet tailored to energy and recovery needs.
- ▪Training: Combine resistance and aerobic activity to complement GH-mediated anabolic and metabolic effects.
- ▪Sleep: Prioritize quality sleep; nocturnal GH secretion is enhanced by adequate rest.
- ▪Timing: Inject fasted or after a 2–3 hour fast; carbohydrate-rich meals may attenuate GHRP-2’s GH response[4].
Potential Benefits & Side Effects
Observations from the literature on each peptide individually; combined-blend evidence is absent, and individual results vary.
Potential Benefits
- ▪Sustained GH / IGF-1: CJC-1295 can sustain elevated GH and IGF-1 over extended periods[1].
- ▪Acute GH pulse: GHRP-2 drives a rapid GH pulse; long-term pediatric studies reported no significant toxicities[2].
- ▪No PCT needed: GH and IGF-1 return to baseline after cessation[1].
- ▪Note on the blend: Synergy of the combination is not established — no clinical trials of this co-formulation exist.
Common Side Effects
- ▪Appetite increase: GHRP-2 may transiently raise appetite via ghrelin-like activity[4].
- ▪Transient hormone shifts: Mild, brief rises in ACTH, cortisol and prolactin may occur after injection[5].
- ▪Flushing / injection-site reactions: CJC-1295 may cause transient facial flushing or lightheadedness; mild local redness can occur[1].
- ▪Sport restriction: Both are WADA-prohibited (S2) at all times.
Injection Technique
General subcutaneous technique, following established clinical best-practice guidance[7][8].
Pre-Injection Preparation
- ▪Wash your hands well with soap and water.
- ▪Wipe the vial stopper with an alcohol swab and let it air-dry.
- ▪Choose a site (abdomen, thigh, or upper arm) and clean it with a fresh alcohol swab, letting it dry fully[8].
- ▪Draw the intended total-blend dose, then check for air bubbles and push any out.
Injection Procedure
- ▪Pinch a skinfold at the chosen site between thumb and forefinger.
- ▪Insert the needle into the pinch at a 45–90-degree angle (use 45 degrees if the fat layer is thin)[7].
- ▪Skip aspiration for subcutaneous shots — it isn’t needed[8].
- ▪Press the plunger slowly and steadily until it’s fully down.
- ▪Wait 5–10 seconds, then pull the needle straight out to prevent leakage.
Post-Injection Care
- ▪Drop the used syringe straight into a puncture-proof sharps container — never recap a needle.
- ▪Return the reconstituted vial to the fridge right away.
- ▪Rotate the injection site each day to prevent irritation and lipohypertrophy.
- ▪Watch the site for excess redness, swelling, or signs of infection.
Recommended Source
For high-purity research peptides, we point researchers to Prime Lab Peptides.
Why Prime Lab Peptides?
- ▪Top-rated on Trustpilot: Independently reviewed as the highest-rated peptide lab on Trustpilot — making it the best current source in the USA.
- ▪Third-party tested: Every batch ships with a Certificate of Analysis (COA) confirming purity and composition.
- ▪Consistent quality: ISO-aligned manufacturing and handling keep product integrity reliable batch to batch.
- ▪Cold-chain integrity: Temperature-controlled shipping and storage across the whole fulfilment chain.
- ▪Research-grade purity: Fit for educational and research use that demands high-quality peptides.
Note: Product availability and specifications subject to change. Verify current product details on supplier website.
References
- 1
J Clin Endocrinol Metab (2006)Teichman SL et al. Prolonged stimulation of GH and IGF-I secretion by CJC-1295, a long-acting GHRH analog, in healthy adults.
- 2
J Clin Endocrinol Metab (1998)Mericq V et al. Effects of eight months of treatment with graded doses of GHRP in GH-deficient children.
- 3
J Clin Endocrinol Metab (1998)Pihoker C et al. Pharmacokinetics and pharmacodynamics of GHRP-2: a phase I study in children.
- 4
J Clin Endocrinol Metab (2005)Laferrere B et al. GHRP-2, like ghrelin, increases food intake in healthy men.
- 5
Peptides (1997)Arvat E et al. Effects of GHRP-2 and hexarelin on GH, prolactin, ACTH, and cortisol levels in man.
- 6
WADA Technical Document (2019)Testing guide for Growth Hormone Releasing Factors (GHRFs), Version 2.0 – GHRH analogs and GH secretagogues prohibited (S2).
- 7
- 8
NCBI BookshelfBest practices for injection (asepsis, preparation, and administration).
- 9
- 10
How to reconstitute CJC-1295 + GHRP-2
- 1Wipe the vial stopper and your bacteriostatic-water vial with an alcohol swab.
- 2Draw your chosen amount of bacteriostatic water and inject it slowly down the inner wall of the CJC-1295 + GHRP-2 vial.
- 3Let it rest, then gently swirl until fully dissolved — do not shake.
- 4Refrigerate the reconstituted vial and draw each dose with a U-100 insulin syringe.
CJC-1295 + GHRP-2 — frequently asked questions
How do I reconstitute a 10 mg vial of CJC-1295 + GHRP-2?
Wipe the stopper with an alcohol swab, then inject your bacteriostatic water slowly down the inside wall of the vial. Let it sit and gently swirl until dissolved — never shake. Store the mixed vial in the refrigerator and draw doses with an insulin syringe. Use the calculator above to turn any dose into syringe units.
How much bacteriostatic water should I add to CJC-1295 + GHRP-2?
There is no single correct amount — more water simply spreads the same 10 mg of peptide across a larger volume, which makes small doses easier to measure accurately. 1 to 3 mL per vial is typical. Enter your chosen volume in the calculator above to see the resulting concentration and syringe units.
What do the "units" on an insulin syringe mean?
On a U-100 insulin syringe, 100 units equal 1 mL, so 1 unit equals 0.01 mL. The calculator above converts your draw volume into these units automatically so you can measure without doing the math by hand.
How should I store CJC-1295 + GHRP-2 after mixing?
Keep the reconstituted vial refrigerated at roughly 2 to 8 degrees Celsius, away from light, and avoid freezing it. Reconstituted research peptides are generally used within a few weeks. Always follow the specific guidance supplied with your product.
How many doses does a 10 mg vial of CJC-1295 + GHRP-2 provide?
Divide the vial strength of 10 mg by the amount you use per injection. The calculator above reports this as "doses per vial" the moment you enter a dose.
Is CJC-1295 + GHRP-2 approved for human use?
No. CJC-1295 + GHRP-2 is sold strictly for laboratory and research purposes and is not approved by the FDA or other regulators for human use. Everything on this page is research information, not medical advice — consult a licensed healthcare professional before any use.
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